P-235: No Association of Endothelial Nitric Oxide Synthase (eNOS) -786T/C Polymorphism with Unexplained Recurrent Abortion in Iranian Women

Background: This is a case-control study to determine the relationship between endothelial nitric oxide synthase (eNOS) gene -786T/C polymorphism in women with unexplaiend recurrent abortion in comparison with healty women.Materials and Methods: 95 women with history of at least 2 unexplaiend recurrent abortion in the reproductive age range 20-35 years as patients group and 95 healty women (age-matched) who had at least one live birth and no history of abortion were chosen. Genotyping of eNOS -786T/C polymorphism was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: Genotype frequencies of eNOS -786T/C polymorphism for the patient group were as follows TT(27.3%),TC (65.2%) and CC (7%) while in control group genotype were 37.8, 50.5 and 11.5%, respectively. Allele frequencies of the eNOS -786T/C polymorphism among patient and control groups were 0.60 and 0.63, respectively for the T allele and 0.40 and 0.36 respectively, for the C allele. Conclusion: Our result demonstrated no association between unexplaiend recurrent abortion and -786T/C polymorphism of eNOS gene in Iranian women.